Protein_Domain
VP64

Part:BBa_J176013:Design

Designed by: Karmella Haynes   Group: Haynes Lab   (2011-09-29)
Revision as of 02:09, 22 November 2011 by Kahaynes (Talk | contribs) (→‎References)

VP64


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TBA


Source

TBA

References

In order of date published:

(1992) Seipel K, Georgiev O, Schaffner W. Different activation domains stimulate transcription from remote ('enhancer') and proximal ('promoter') positions. EMBO J. 1992 Dec;11(13):4961-8.
Note: Earliest reported test of a "modular" VP16 "core" activator, DALDDFDLDML; activity was compared to activation domains from other transcription factors

(2005) Dreier B, Fuller RP, Segal DJ, Lund CV, Blancafort P, Huber A, Koksch B, Barbas CF 3rd. Development of Zinc Finger Domains for Recognition of the 5′-CNN-3′ Family DNA Sequences and Their Use in the Construction of Artificial Transcription Factors. J Biol Chem. 2005 Oct 21;280(42):35588-97.
Note: VP16 tetramer VP64 ([DALDDFDLDML]-GS-[DALDDFDLDML]-GS-[DALDDFDLDML]-GS-[DALDDFDLDML]) fused to a zinc finger; synthetic construct added to NCBI database (GenBank: AAZ80416.1) in 2005