Part:BBa_K672000:Experience

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Please enter how you used this part and how it worked out.

Applications of BBa_k672000

Able to sense nitrate and nitrites even after experiencing a cold shock at 20ºC or below.

Experience with BBa_K672000

FIRS ROUND OF EXPERIMENTATION

We designed the following experiment in order to: test the BioBrick operation in different substrates and temperatures, characterize the expression of the promoters, determine the strongest promoter for the device operation, define the most efficient temperature and substrate for the operation of this device, provide a way to survive at low temperatures and be able to measure nitrate.

We chose 4 temperatures:

• 5-10°C

• 10-20°C

• 20-30°C

• 37°C

These temperatures were selected so we could evaluate the response to cold shock in various ranges for our BioBrick. Since the lab equipments we had available couldn’t reach temperatures between 10-20°C this temperature was not used during the experience. In the end, the lab equipment allowed us to make the experiments in: 37°C, 23°C and 8°C.

We also chose 3 different Growth Media:

• Saline Solution

• Liquid LB

• Minimal Media

These growth medias where selected to evaluate the response of our BioBricks in different metabolic pathways.

We did Spectrophotometry measurments, obtaining the following results:

LB stands for Luria Broth; MM, for Minimal Media and SS, for Saline Solution.

Which leads us to the following conclusions:

1. The best growth is obtained in Luria Broth.
   
2. Even though we obtained some growth at 23°C in the LB substrate, the Biobrick doesn't fulfill our expectations and requirements.

SECOND ROUND OF EXPERIMANTATION

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