![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K649300
Designed by: Goshi Sugano Group: iGEM11_Tokyo_Tech (2011-09-19)
Revision as of 12:04, 28 October 2011 by Notothenia (Talk | contribs)
Arginase (rocF)
This part has only rocF obtained from B.subtilis 168 by colony-PCR.
rocF is the cording region of Arginase(EC:3.5.3.1), catalyzing the reaction from arginine and water to urea and ornithine.
Original rocF has two EcoRI sites in ORF, so we changed these sites.
To characterize this part, we constructed the composite part(BBa_K649301). To know detailed infomation, please click here.
Introduction of BBa_K649301 containing arginase coding gene (rocF) led to production of some urea in strain MG1655 (argR +).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 137
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |