Plasmid_Backbone

Part:BBa_K560016

Designed by: Zhang Chao   Group: iGEM11_WHU-China   (2011-10-26)
Revision as of 12:16, 26 October 2011 by Registry (Talk | contribs)

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BsaI Golden Gate Assembly_Backbone

N

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 644
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 35
    Illegal NheI site found at 2776
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XhoI site found at 363
    Illegal XhoI site found at 1797
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 644
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 644
    Illegal NgoMIV site found at 1650
    Illegal AgeI site found at 569
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 575
    Illegal BsaI.rc site found at 128
    Illegal SapI.rc site found at 2653


[edit]
Categories
Parameters
n/aBsaI Golden Gate Assembly_Backbone