Composite

Part:BBa_K590030

Designed by: Matthew Harger, Lei Zheng   Group: iGEM11_Washington   (2011-09-15)
Revision as of 20:49, 24 October 2011 by Hargem (Talk | contribs)

DrR-FabH2-PSB1C3-High constitutive

This part consists of Aldehyde Decarbonylase, from Synechococcus elongatus, ribosomal binding site, Acyl-ACP Reductase, and FabH2 in PSB1C3 high consititutive vector for even chain length alkaneproduction. This construct is designed to produce branched odd chain alkanes. Use of this part results in low alkane yields ( 10mg/L), and results in slow cell growth. FabH2 should be used on a low copy number inducible vector. For more information, refer to [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team's wiki].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 470
    Illegal NheI site found at 493
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1254
    Illegal XhoI site found at 2366
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
    Illegal AgeI site found at 2728
    Illegal AgeI site found at 3367
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2773


[edit]
Categories
Parameters
None