Generator

Part:BBa_K606010

Designed by: Cyrille Pauthenier   Group: iGEM11_Paris_Bettencourt   (2011-07-07)
Revision as of 22:16, 25 October 2011 by Cyrpaut (Talk | contribs)

LacI strong expression casette for B. Subtilis

This part is designed to produce an expression of LacI in the B. Subtilis cell, in order to control the expression of the promoter pHyperSpank https://parts.igem.org/Part:BBa_K606040 K1606040] level of expression.


Usage and Biology

LacI gene is not expressed enough in B. Subtilis to be sufficient to regulate strongly the pHyperSpank promoter. This is a sub-part of the IPTG inducible expression cassette K606011 for Bacillus Subtilis, which contains the complete system

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/regulatory/uncategorized
Parameters
negative_regulatorspHyperSpank