Reporter

Part:BBa_K606063:Design

Designed by: Camille de Froberville   Group: iGEM11_Paris_Bettencourt   (2011-10-23)
Revision as of 17:07, 23 October 2011 by Kyauy (Talk | contribs) (→‎Design Notes)

T7 amber expression monitored by a linear system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 917
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 693


Design Notes

To design the T7 amber we had to consider how many amber sites we would put there for it to be the less leaky possible. Our choice is 2 mutations Amber.

Source

The T7 amber polymerase is from the classic T7 polymerase (part BBa_K145001).

References