Coding
Part:BBa_K633002
Designed by: Ricardo Camilo Chavez Martinez Group: iGEM11_Tec-Monterrey (2011-09-27)
RBS+signal peptide phoA+Cellulase
We used Rybosome Binding Site BBa_B0034 designed by by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr, evaluated by Warsaw's 2010 team. we added membrane protein signal peptide phoA and cellulase immediately next to it
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1908
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 721
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 585
Illegal AgeI site found at 228
Illegal AgeI site found at 644
Illegal AgeI site found at 1473 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//function/degradation/cellulose
None |