Generator

Part:BBa_K559010

Designed by: Jacky, Fong Chuen, Loo   Group: iGEM11_Hong_Kong-CUHK   (2011-09-29)
Revision as of 22:29, 14 October 2011 by Jacky Loo (Talk | contribs)

Halorhodopsin complete system

Halorhodopsin gene,light-driven ion pump, allow chloride ion to be pumped in by the light input. The mechanism can be refered to BBa_K559000..

Halorhodopsin gene is under regulation by constitute T7 promoter to allow protein coding to be maximized. The details can be refered to BBa_K559001.

Bidirectional terminator is added for high efficiency termination of both forward and backward transcription

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 368
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 380
    Illegal AgeI site found at 106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 316


[edit]
Categories
//cds/membrane/channel
//chassis/prokaryote/ecoli
//function/sensor/light
//plasmid/expression/t7
Parameters
biology
device_type
function
protein