Generator

Part:BBa_K624002

Designed by: CHUN-YU LAI   Group: iGEM11_NYMU-Taipei   (2011-09-30)
Revision as of 12:39, 14 October 2011 by Ray0201 (Talk | contribs)

pT7+RBS+tetR+RBS+GFP+Ter

TetR and GFP genes are both under pT7 promoter control. However, pT7 can only function uder the presence of associated refulator, thus, for example, when we transform this construct into strain BL21 E. coli, which express associated regulator, both tetR and GFP will be expressed.In contrast, if we transform this construct into other strain without regulatory protein both genes will not expressed. Due to that tetR and GFP is under sthe regulation of the same promoter, we can quantitize the tetR expression by detection of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1430


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Categories
Parameters
None