Composite

Part:BBa_K633014

Designed by: Ricardo Camilo Chavez Martinez   Group: iGEM11_Tec-Monterrey   (2011-09-27)
Revision as of 05:46, 29 September 2011 by Bbm (Talk | contribs)

AraC+Pbad promoter+RBS+signal peptide phoA+Cellulase+Linker+estA membrane protein

The composite part includes a functional expression vector for autotransporter membrane protein estA, with a linker united to a cellulase, attached to a membrane signaling peptide. This was our final construct missing a terminator

The construct efectiveness was tested by an enzymatic assay, The results can be seen here: https://static.igem.org/mediawiki/2011/8/8b/Roseta_results.pdf

More detailed results: http://2011.igem.org/Team:Tec-Monterrey/projectresults


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1247
    Illegal NheI site found at 3168
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1187
    Illegal BamHI site found at 1981
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1845
    Illegal NgoMIV site found at 3398
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1488
    Illegal AgeI site found at 1904
    Illegal AgeI site found at 2733
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
//cds/enzyme
//cds/membrane
//function/degradation/cellulose
Parameters
None