Part:BBa_I15010:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I15010

This part was used by the 2009 WashU iGEM team[http://2009.igem.org/Team:Wash_U] as an integral part of their light sensitive regulation of LHII antennae size in Rhodobacter sphaeroides. However, due to its uncertain reliability in the Registry (a PstI cut site still exists in the part), manipulation of DNA containing the part was necessarily limited. The WashU team is attempting to fix and resubmit this part to the registry.

The 2011 University of Minnesota iGEM team was unable to transform this part into E. coli. Two attempts were made to transform this part with known good competent cells. Competent cells were known to be good because other parts were simultaneously transformed with these cells.

User Reviews

UNIQ6ec49187ed425c96-partinfo-00000000-QINU

This part was sent to WashU iGEM as an agar slab and it is not of decent quality, as suggested in the Registry quality control information. We are in the process of attempting to resubmit the part.

This review comes from the old result system and indicates that this part did not work in some test.

UNIQ6ec49187ed425c96-partinfo-00000002-QINU

iGEM11_Uppsala-Sweden

This part was used by the 2011 Uppsala-Sweden team[http://2011.igem.org/Team:Uppsala-Sweden] as an integral part of the multichromatic light sensing project. Repeated attempts to transform or PCR the DNA from the 2011 iGEM distribution kit failed while all other parts seemed viable. Uppsala-Sweden requested the Jeffrey Tabor's pJT122 plasmid from Chris A. Voigt and amplified the cph8 DNA from there. Tabor's cph8 sequenced contained one internal PstI site, which was subsequently removed. The resultant DNA sequence was identical to that of BBa_I15010. Uppsala-Sweden sequenced this identical DNA part and submitted it with a new BioBrick number: BBa_K592000. Furthermore, Uppsala-Sweden also submitted the part BBa_K592018 which is Cph8 with the BBa_B0034 ribosome binding site. This version of Cph8 with RBS has been completely sequenced and submitted to the registry.

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iGEM11_WHU_China

This part was used by 2011 WHU_China team as a part of light sensing system. However, the result of sequencing proves that this part in the 2011 kit turns out to be a vector without the fragment of cph8. 2011 WHU_China requested the same part from the 2011 kit of HUST_China, but the result of pcr and restriction enzymes cleavage shows that their plasmids have the same problem.

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