Reporter

Part:BBa_K676013:Design

Designed by: Meng Li   Group: iGEM11_UCL_London   (2011-09-19)
Revision as of 21:55, 21 September 2011 by Khengtee (Talk | contribs) (→‎Source)

Mu/Spy Ligation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 191
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1203


Design Notes

AFter the PCR and ligation of Mu GBS into a pSB1C3 backbone, we carried out 3A Assembly of the Mu GBS with the SPY device in BBa_K239009 into a pSB1A3

Source

2009 UCL SPY device

References