Cell

Part:BBa_K624032:Experience

Designed by: Shang-Jui Tsai   Group: iGEM11_NYMU-Taipei   (2011-10-04)
Revision as of 07:12, 9 October 2011 by Lchieh72 (Talk | contribs) (→‎Applications of BBa_K624032)

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Applications of BBa_K624032

We measure AMB-1's growth under OD565, which is the best absorption wavelength for AMB-1. For electroporation operation, it is important to find out the log phase, since it’s the best time for electroporation. By keeping AMB-1 in "log phase" (approximately 3~6 days in Enrich-MSGM, 7~10 days in MSGM), best results of electroporation are achieved. And after seven days, the bacterium would go into stationary phase. During the phase, the growth slows as a result of nutrient depletion and accumulation of toxic products. Growth rate and death rate of bacterium is best likely to meet equilibrium in this stage.

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