Generator

Part:BBa_K624057

Designed by: Yi-Hong Liou   Group: iGEM11_NYMU-Taipei   (2011-10-05)
Revision as of 11:20, 9 October 2011 by Peichinghuang (Talk | contribs)

pYMB essentials + RBS(Pmsp3) + LLO

pYMB essentials + RBS(Pmsp3) + LLO

pYMB is a shuttle vector for manipulating and transforming gene into AMB-1 RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter of AMB-1 LLO (Listeriolysin O) can make the bacteria be able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. which help protect the AMB-1 from extracellular immune system. The ribosome binding site is from Pmsp3.(BBa_K624012).

The ribosome binding site is from Pmsp3.(BBa_K624012).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2567
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None