Part:BBa_K624032

Magnetospirillum magneticum AMB-1

Facultative anaeroabic magnetotactic bacterial species, Magnetospirillum magneticum AMB-1. Grow in liquid MSGM (Magnetic Spirillum Growth Medium), or on MSGM agar plate.Like E. coli, AMB-1 also grows in liquid medium, and this medium is called Magnetic Spirillum Growth Medium (MSGM). In the medium, we added nutrient, iron source (supplying AMB-1 in magnetite formation within magnetosomes), vitamin, and mineral solution.

MSGM's ingredients

(In grams per liter)

●Succinic acid             0.74 g
●KH2PO4                    0.68 g
●Sodium nitrate            0.12 g
●Sodium thioglycolate      0.1 g
●[http://2011.igem.org/File:Wolfe_vitamin.pdf *Wolfe's vitamin solution]  10 ml(stock)
●[http://2011.igem.org/File:Wolfe.pdf *Wolfe’s mineral solution]  5  ml(stock)
●Ferric quinate solution   2  ml(0.27 g of FeCl3 and 0.19 g of quinic acid in 100 ml water)
 Adjust pH to 7.0 with NaOH

NCBI: [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=genome&Cmd=ShowDetailView&TermToSearch=19021 whole genome sequence]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]