Composite

Part:BBa_K539741:Design

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Formosa   (2011-09-22)
Revision as of 10:40, 8 October 2011 by Bettyyang (Talk | contribs) (→‎Design Notes)

TetR repressible promoter (BBa_R0040)+ kivd (alpha-ketoisovalerate decarboxylase)+Ter (BBa_J61048)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 658
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1735
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Kivd is under tetR repressible promoter that is continuously turn ON or repressed by tetR.

Source

Kivd is cloned form Lactococcus lactis subsp. lactis KF147

References