Coding

Part:BBa_K539711

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Formosa   (2011-09-20)
Revision as of 09:32, 8 October 2011 by Bettyyang (Talk | contribs)

ilvC (ketol-acid reductoisomerase NAD(P)-binding)

This is the coding region of ilvC from Escherichia coli str. K-12 substr(MG1655). It encodes ketol-acid reductoisomerase NAD(P)-binding which is part of the isobutanol biosynthesis pathway. Following is the isobutanol biosynthesis pathway.
Pathway%28small%29.jpg
Our team has mutated the function of RE site within the original ilvC .
Ilvc.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 592
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 178
    Illegal BsaI.rc site found at 997


[edit]
Categories
Parameters
None