Composite

Part:BBa_K539742:Design

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Formosa   (2011-09-22)
Revision as of 06:34, 7 October 2011 by Bettyyang (Talk | contribs) (→‎Design Notes)

TetR repressible promoter (BBa_R0040)+ kivd (alpha-ketoisovalerate decarboxylase)+Ter (BBa_B0015)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 658
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene Kivd is cloned from Escherichia coli str. K-12 substr(MG1655) by our team, NCTU-Formosa. We also modify the RE site in the coding sequencing.

Source

It is cloned form Lactococcus lactis subsp. lactis KF147

References