Part:BBa_S01010:Experience

Applications of BBa_S01010

We use this part in the device 2 in our team OUC-China in 2011( please see http://2011.igem.org/Team:OUC-China/Result/fv), However, when we ligate it with K084005, B0015, R0079. We see that the DNA length is much longer than we expected. The electrophoretogram is as below. The S01010 can act as a cinI AHLs gnerator with reporter ECFP, It can paly an important role in our device 2 in our team OUC-China in 2011,saving us a lot of time, as shown in http://2011.igem.org/Team:OUC-China/Result/fv,

User Reviews

UNIQ9eb0594d9e720352-partinfo-00000000-QINU

Don Quixia

This review comes from the old result system and indicates that this part did not work in some test.

UNIQ9eb0594d9e720352-partinfo-00000002-QINU We use this part in the device 2 in our team OUC-China in 2011( please see http://2011.igem.org/Team:OUC-China/Result/fv), However, when we ligate it with K084005, B0015, R0079. We see that the DNA length is much longer than we expected. The electrophoretogram is as below.

The K084005, B0015, R0079 and S01010 (18-3-11-16) are supposed to add up to 3000, however the electrophoretogram result above shows that they surpass 3000 greatly.

then we sequence the S01010, find that this part is of quality problems indeed. It proves that S01010 is 3094bp (longer than its expected length 1631bp)and its actual sequence is as below,
TACTAGTAGCGGCCGCTGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTCG
TTACCTATAAAATACGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCT
TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTATGACAACTTGACGG
CTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTG
CATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGAC
GGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGG
TCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGAC
GCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGC
CAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGA
GCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCC
AGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTC
GCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATT
GACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGT
GATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAA
CAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCT
GACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAA
AAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAA
ACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCG
CCATTCAGAGAAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTT
TTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAAC
AAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCA
GAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTAT
CCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCAT
ACCGTTTTTTTGGGCTAGCTACTAGAGAAAGAGGAGAAA(RBS)TACTAGATGACAGTA
AAGAAGCTTTATTTCGTCCCAGCAGGTCGTTGTATGTTGGATCATTCGTCTGTTAATAGT
ACATTAACACCAGGAGAATTATTAGACTTACCGGTTTGGTGTTATCTTTTGGAGACTGA
AGAAGGACCTATTTTAGTAGATACAGGTATGCCAGAAAGTGCAGTTAATAATGAAGGTC
TTTTTAACGGTACATTTGTCGAAGGGCAGGTTTTACCGAAAATGACTGAAGAAGATAG
AATCGTGAATATTTTAAAACGGGTTGGTTATGAGCCGGAAGACCTTCTTTATATTATTAG
TTCTCACTTGCATTTTGATCATGCAGGAGGAAATGGCGCTTTTATAAATACACCAATCAT
TGTACAGCGTGCTGAATATGAGGCGGCGCAGCATAGCGAAGAATATTTGAAAGAATGTA
TATTGCCGAATTTAAACTACAAAATCATTGAAGGTGATTATGAAGTCGTACCAGGAGTT
CAATTATTGCATACACCAGGCCATACTCCAGGGCATCAATCGCTATTAATTGAGACAGA
AAAATCCGGTCCTGTATTATTAACGATTGATGCATCGTATACGAAAGAGAATTTTGAAAA
TGAAGTGCCATTTGCGGGATTTGATTCAGAATTAGCTTTATCTTCAATTAAACGTTTAAA
AGAAGTGGTGATGAAAGAGAAGCCGATTGTTTTCTTTGGACATGATATAGAGCAGGAA
AGGGGATGTAAAGTGTTCCCTGAATATATAGCTGCAAACGACGAAAACTACGCTTTAGT
AGCTTAATAACGCTGATAGTGCTAGTGTAGATCGCTACTAGAGAAAGAGGAGAAA(RBS)
TACTAGATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCG
AGCTGGACGGCGACGTGAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGC
GATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCG
TGCCCTGGCCCACCCTCGTGACCACCCTGACCTGGGGCGTGCAGTGCTTCAGCCGCTA
CCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTC
CAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTG
AAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAG
GAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACATCAGCCACAACGTC
TATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACA
ACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCG
GCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGA
GCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCG
CCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAATAATACTAGAGCCAGGCATC
AAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTC
GGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTT
ATA(E0020+B0015, ECFP+double teminator)
TACTAGTAGCGGCCGCTGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTCG

Through sequence analysis, we can see that the S01010 has lost its necessary part C0076 and only includes RBS, E0020 and B0015 in the end. Its former sequence has been wrong, this may affect its E0020's expression, so no ECFP is expressed in device II when detected under fluorescence microscope.