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Translational_Unit

Part:BBa_K567012

Designed by: Bin Zhao   Group: iGEM11_SJTU-BioX-Shanghai   (2011-09-29)
Revision as of 02:57, 6 October 2011 by Alfred (Talk | contribs)

tRNA(Asp)-AGG

tRNA(Asp) with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of aspV promoter. This biobrick is constructed first by cloning the tRNA(Asp) from aspV in E.coli, then the anticodon region is site-directed mutated.

Get more information from Biobrick PT7-TDRS (BBa_K567011)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 281
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 281
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 281
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 281
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 281
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
chassisEscherichia coli