Composite

Part:BBa_K567001

Designed by: Chunying Li and Yunfeng Ruan   Group: iGEM11_SJTU-BioX-Shanghai   (2011-09-29)
Revision as of 03:10, 6 October 2011 by Alfred (Talk | contribs)

lacI-Ptrc-tRNA(Arg)

The tRNA(Arg) is under the control of promoter trc. tRNA(Arg) expression is induced by 0.5mM IPTG when the OD600 of the culture reaches 0.3.

Related Biobrick:

Pbla-Luc-2AGG (BBa_K567004)

Pbla-Luc-4AGG (BBa_K567005)

Pbla-Luc-6AGG (BBa_K567006)

Pbla-Luc-8AGG (BBa_K567007)

PT7-Luc-2AGG (BBa_K567008)

PT7-Luc-4AGG (BBa_K567009)

PT7-Luc-6AGG (BBa_K567019)

PT7-Luc-8AGG (BBa_K567010)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1845
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1845
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1845
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1845
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2026


[edit]
Categories
Parameters
chassisEscherichia coli
functionIPTG induced tRNA expression
ligandsIsopropyl β-D-1-Thiogalactopyranoside
n/alacI-Ptrc-tRNA(Arg)