Coding
trkD

Part:BBa_K629005

Designed by: Zilong WANG, Yi ZHENG   Group: iGEM11_SYSU-China   (2011-10-04)
Revision as of 08:29, 4 October 2011 by Registry (Talk | contribs)

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trkD, a functional Kup (formerly TrkD) system took up Cs+ with a moderate rate and affinity

Escherichia coli cells which contain a functional Kup (formerly TrkD) system took up Cs' with a moderate rate and affinity. Kup is a separate K+ uptake system with relatively little discrimination in the transport of the cations K+, Rb+, and Cs'. Regardless of the presence or absence of Kup, K+-replete cells took up Cs'primarily by a very low affinity mode, proportional to the ratio of the Cs' and K+ concentrations in the medium. In our project, trkD is used to collect Cs-137 when recN is activated by radiation and start the transcription of trkD,in order to absorb this kind of radioactive source like Cs-137

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 245
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1320


[edit]
Categories
//cds/membrane/transporter
//chassis/prokaryote/ecoli
//classic/other/uncategorized
//proteindomain/transmembrane
//ribosome/prokaryote/ecoli
//terminator/single
Parameters
efficiency143.478% relative absorption of cesium-137 to control
functioncesium absorption
n/atrkD, a functional Kup (formerly TrkD) system took up Cs+ with a moderate rate and affinity
origingenome of E. Coli BL21 Plys