RNA

Part:BBa_K581003

Designed by: Chen Yiwei, Qin Xiao   Group: iGEM11_Peking_S   (2011-09-26)
Revision as of 22:12, 5 October 2011 by Chenmiaomiao (Talk | contribs)

SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)

This is the conjugate part of ptsG2-gfp.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


This BioBrick has been sequence verified.

Background

SgrS is an Hfq-binding small antisense RNA that is induced upon phosphosugar stress (Vanderpool, 2007). It forms a ribonucleoprotein complex with RNase E through Hfq to mediate silencing of the target ptsG mRNA encoding the major glucose transporter (Geissmann and Touati, 2004). A 31-nt-long stretch in the 3’ region of SgrS is partially complementary to the translation initiation region of ptsG mRNA, and a 6 nt region overlapping the Shine-Dalgarno sequence of the target mRNA turns out to be crucial for SgrS’ function, shown as Fig 1 (Kawamoto et al., 2006; Maki et al., 2010).

[[File:M_Mechanism.png‎|680px]] [[File:M_P1_S1.png|680px]] [[File:M_P2_S2.png‎|680px]] ''Fig. 2 Sequence alignment of wild type ptsG/SgrS pair and its mutant complementary pairs.'' ''(A) The partial complementary region of ptsG (wt) mRNA and its corresponding sRNA SgrS.'' ''(B) The complementary pair of ptsG1 mRNA and corresponding SgrS1.'' ''(C) Another complementary pair site-mutant version of ptsG2 mRNA and corresponding SgrS2.''

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Experimental Data

Methods

References

[1] Geissmann, T.A., and Touati, D. (2004). Hfq, a new chaperoning role: binding to messenger RNA determines access for small RNA regulator. The EMBO journal 23: 396-405

[2] Kawamoto, H., Koide, Y., Morita, T., and Aiba, H. (2006). Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq. Molecular microbiology 61: 1013-1022

[3] Levine, E., Zhang, Z., Kuhlman, T., and Hwa, T. (2007). Quantitative characteristics of gene regulation by small RNA. PLoS biology 5: e229

[edit]
Categories
//chassis/prokaryote/ecoli
//regulation/negative
Parameters
device_typepost-transcriptional regulators of gene expression
familyHfq-binding small RNA
functiondestabilzes ptsG2 mRNA
genotypeG176C mutant of SgrS(wt)
n/aSgrS2 (conjugate part of ptsG2 in comparator)
originE.coli K-12 strain
target5' Untranslated Regions