Translational_Unit
Part:BBa_K578005
Designed by: Kylie Standage-Beier Group: iGEM11_Arizona_State (2011-09-28)
J23102+E0840 (Constitutive GFP)
This is a combination of the GFP coding region (E0840), and the medium strength constitutive promoter, J23102.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 708
Confirmation of Function
This BioBrick was successfully transformed into a variety of vectors, including pSB1C3, pSB1A3, pSB1K3, and Novagen pRSF Duet. Success was evident due to the presence of green fluorescent colonies.
Use in Competent Cells
E. Coli MG1655 with this BioBrick in pRSF Duet was successfully made chemically competent using the OWW TSS procedure, allowing for the transformation of a second plasmid.
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Categories
Parameters
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