RNA

Part:BBa_K581004

Designed by: Chen Yiwei, Qin Xiao   Group: iGEM11_Peking_S   (2011-09-26)
Revision as of 22:08, 5 October 2011 by QINXIAO (Talk | contribs)

ptsG2-GFP (ptsG2 5'UTR fused with gfp)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 747


This BioBrick has been sequence verified.

Background

'''ptsG2''' is the C87G mutant of ptsG(wt) and the conjugate part of SgrS2 in our comparator device (see Fig. 1). [[Image:MM P2 S2.png]] ''Fig. 1 The complementary pair of ptsG2 mRNA and corresponding SgrS2.'' PtsG is a glucose permease which is subordinate to phosphotransferase system and serves as a transporter. Here, this mRNA performs as the conjugate part of the small RNA regulator sgrS(wt). ptsG mRNA is regulated by SgrS by short, imperfect base-pairing interactions, and its expression is thus repressed.

Experimental Data

Methods

References

[1] Geissmann, T.A., and Touati, D. (2004). Hfq, a new chaperoning role: binding to messenger RNA determines access for small RNA regulator. The EMBO journal 23: 396-405

[2] Kawamoto, H., Koide, Y., Morita, T., and Aiba, H. (2006). Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq. Molecular microbiology 61: 1013-1022

[3] Levine, E., Zhang, Z., Kuhlman, T., and Hwa, T. (2007). Quantitative characteristics of gene regulation by small RNA. PLoS biology 5: e229

[edit]
Categories
//chassis/prokaryote/ecoli
transcriptional
Parameters
biology5' regulatory region of ptsG2 is the target of sRNA SgrS2
chassisE. coli DH5α
genotypeC87G mutant of ptsG(wt)
n/aptsG2 (conjugate part of SgrS2 in comparator)