Coding

Part:BBa_K572005:Design

Designed by: Avaneesh T   Group: iGEM11_IIT_Madras   (2011-09-18)
Revision as of 18:26, 18 September 2011 by Chai.1190 (Talk | contribs) (→‎Design Notes)

Proteorhodopsin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene had E,S and P sites. Thus, we used site directed mutagenesis to optimize the codon and to remove the E,S and P sites so that it can be ligated with the iGEM plasmid backbone.

Source

Source Organism : Marine gamma proteobacterium EBAC31A08.

We would like to thank Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea for sending us the plasmid (pKJ900) with proteorhodopsin gene.

References

Martinez A, Bradley AS, Waldbauer JR, Summons RE, DeLong EF (2007). "Proteorhodopsin photosystem gene expression enables photophosphorylation in a heterologous host". PNAS 104 (13): 5590–5595. Bibcode 2007PNAS..104.5590M. doi:10.1073/pnas.0611470104. PMC 1838496. PMID 17372221.