RNA

Part:BBa_K581003

Designed by: Chen Yiwei, Qin Xiao   Group: iGEM11_Peking_S   (2011-09-26)
Revision as of 21:02, 5 October 2011 by Chenmiaomiao (Talk | contribs)

SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)

This is the conjugate part of ptsG2-gfp.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


This BioBrick has been sequence verified.

Background

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[[Image:Mechanism.png]]

Figure 1: Sequence alignment of wildtype ptsG/SgrS pair and its mutant complementary pairs.


References

[1] Geissmann, T.A., and Touati, D. (2004) Hfq, a new chaperoning role: binding to messenger RNA determines access for small RNA regulator. The EMBO journal 23: 396-405

[2] . :

[3] . :.

[edit]
Categories
//chassis/prokaryote/ecoli
//regulation/negative
Parameters
device_typepost-transcriptional regulators of gene expression
familyHfq-binding small RNA
functiondestabilzes ptsG2 mRNA
genotypeG176C mutant of SgrS(wt）
n/aSgrS2 (conjugate part of ptsG2 in comparator)
originE.coli K-12 strain
target5' Untranslated Regions