Device

Part:BBa_K598019

Designed by: Zhenrun ZHANG   Group: iGEM11_Peking_R   (2011-09-27)
Revision as of 15:25, 27 September 2011 by Registry (Talk | contribs)

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B0015+pBAD+vioABD+B0015+pBAD+TPP Down-regulated Hammerhead Ribozyme 2.5+vioE

B0015+pBAD+vioABD+B0015+pBAD+TPP Down-regulated Hammerhead Ribozyme 2.5+vioE

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 262
    Illegal NheI site found at 1296
    Illegal NheI site found at 5973
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 202
    Illegal BamHI site found at 5913
    Illegal BamHI site found at 6105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4081
    Illegal AgeI site found at 4277
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 5506
    Illegal SapI.rc site found at 5581


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//plasmidbackbone/copynumber/high
//regulation/negative
Parameters
chassisE. coli
ligandsThiamine Pyrophosphate (TPP)
resistance chloramphenicol