Composite
Part:BBa_K544003
Designed by: Leung Man Hong Group: iGEM11_HKU-Hong_Kong (2011-09-30)
Revision as of 19:22, 5 October 2011 by GeorgeLeung (Talk | contribs)
Tetracycline Repressor with Consitutive Promoter BBa_J23116
A transcriptional regulator TetR (BBa_C0040) is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106), which can be used to tune the expression level of constitutively expressed TetR.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 65
Illegal SpeI site found at 37 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 37 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 65
Illegal SpeI site found at 37 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 65
Illegal SpeI site found at 37 - 1000COMPATIBLE WITH RFC[1000]
(The data collected are considered only when difference between values with the P-value <0.05)
(The data collected are considered only when difference between values with the P-value <0.05)
[EGFP: Enhanced Green Fluorescent Protein, sfGFP: Superfolder Green Fluorescent protein ]
[edit]
Categories
Parameters
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
//function/regulation/transcriptional
None |