Composite

Part:BBa_K544002

Designed by: Leung Man Hong   Group: iGEM11_HKU-Hong_Kong   (2011-09-30)
Revision as of 18:49, 5 October 2011 by WeN Dy (Talk | contribs)

Tetracycline Repressor with Consitutive Promoter BBa_J23109

A transcriptional regulator TetR (BBa_C0040) is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106), which can be used to tune the expression level of constitutively expressed TetR.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 65
    Illegal SpeI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 37
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 65
    Illegal SpeI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 65
    Illegal SpeI site found at 37
  • 1000
    COMPATIBLE WITH RFC[1000]


Table 1.jpg
Our team leader is demonstrating the pipetting techniques to the high school students.


Fluorescent intensity of MG1655 -tetO2-0-sfGFP strain after transformation of various repressors (The data collected are considered only when difference between values with the P-value <0.05)



Table 2.jpg
Our team leader is demonstrating the pipetting techniques to the high school students.


Fluorescent intensity of MG1655 -tetO2-0-EGFP strain after transformation of various repressors (The data collected are considered only when difference between values with the P-value <0.05) [EGFP: Enhanced Green Fluorescent Protein, sfGFP: Superfolder Green Fluorescent protein ]


[edit]
Categories
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
Parameters
None