DNA

Part:BBa_K524000

Designed by: HO Yuan Heng Trevor   Group: iGEM11_HKUST-Hong_Kong   (2011-09-15)
Revision as of 19:42, 3 October 2011 by Hyht2011 (Talk | contribs)

Heat sensitive origin of replication (oriR101 & repA101-ts)

oriR101 is an low copy origin of replication that is initiated by the protein repA101. repA101-ts, a mutated form of repA101 would denature at temperatures above 37°C. Hence plasmids using this construct to drive replication can only initiate replication when cultured below 37°C (recommended <30°C). Production of repA101-ts is drived by an unknown constitutive promoter included in the construct.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1709
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None