Composite

Part:BBa_K544013

Designed by: Leung Man Hong   Group: iGEM11_HKU-Hong_Kong   (2011-09-30)
Revision as of 09:18, 30 September 2011 by Registry (Talk | contribs)

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tetR-HNS (2-90) fusion protein (Consitutive Promoter: BBa-J23109)

The tetR-HNS2-90 protein fuses the polymerization demain of histone-like nucleoid-structuring (H-NS) protein to tetR. Thus, in E.coli, when TetR-HNS2-90 binds the TetR-specific DNA motif, it will recruit native H-NS to bind to adjacent DNA, which may lead to local gene silencing. This fusion protein is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106). Experimental data indicates that TetR-HNS2-90 is more effective than TetR on transcription repression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 65
    Illegal SpeI site found at 37
    Illegal PstI site found at 851
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 37
    Illegal PstI site found at 851
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 65
    Illegal SpeI site found at 37
    Illegal PstI site found at 851
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 65
    Illegal SpeI site found at 37
    Illegal PstI site found at 851
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 833


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