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Part:BBa_K539691:Experience

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)
Revision as of 08:13, 5 October 2011 by Zcc (Talk | contribs) (→‎Applications of BBa_K539691)

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Applications of BBa_K539691

Experience by 2011 iGEM NCTU_FORMOSA

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Figure.1 Firstly , we ligase B0030 with ilvC.


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Figure.2 At the sametime , we ligase B0030 with ilvD.


Butanol figure3.jpg

Figure.3 Meanwhile , we ligase B0032 with Alss.


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Figure.4 To make sure we obtain the correct part , colony PCR is needed.


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Figure.5 Secondly , we ligase Plac with B0032+Alss


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Figure.6 Thirdly , we ligase Plac+B0032+Alss with B0030+ilvC


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Figure.7 At the same time , we ligase B0030+ilvD with RNA thermometer+tetR+double terminator


Butanol figure8.jpg

Figure.8 Finally , we ligase the whole circuit:promoter(lacI regulated)+Alss+ilvC+ilvD(each preceded by own RBS)and RNA thermometer+terminator

To make sure our design could work , we cotransform Part:BBa_K539691 and Part:BBa_K539742 into DH5α, so the expression of alss, ilvC, ilvD and kivd are under the control of temperature.

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