Coding

Part:BBa_K533006:Design

Designed by: ZHANG Yunxiao   Group: iGEM11_Tsinghua   (2011-09-29)
Revision as of 14:47, 29 September 2011 by Registry (Talk | contribs)

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Multi-Proline-mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 109
    Illegal BamHI site found at 908
    Illegal XhoI site found at 113
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

proline rich sequence is much longer than the length required for SH3 binding, for easier binding to SH3 domain.


Source

mCherry sequence is from Clontech plasmid pmCherry-N1 and multi-proline sequence is synthesized from a consensus sequence of SH3 binding sequence.

References