Device

Part:BBa_K533005:Experience

Designed by: ZHANG Yunxiao   Group: iGEM11_Tsinghua   (2011-09-29)
Revision as of 15:35, 5 October 2011 by Wishyx (Talk | contribs) (→‎Applications of BBa_K533005)

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Applications of BBa_K533005

This is a surface marker for E. coli, although it can serve other purposes.

We verified its membrane location by different means.


In order to determine whether GFP is presented outside the membrane, cells were digested with Protease K at 50 centigrade for 30min.

Color Channel Post-digest Pre-digest
GFP Thuexp gn.png Thuexp gp.png
DAPI Thuexp gn d.png Thuexp gp d.png
Merge Thuexp gn m.png Thuexp gp m.png

The fluorescence disappeared after digesion, suggesting that the majority of GFP is on the outside of E. coli.

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UNIQ93d3a3eb62416348-partinfo-00000000-QINU UNIQ93d3a3eb62416348-partinfo-00000001-QINU