Generator

Part:BBa_K543015

Designed by: Naoto Shimada   Group: iGEM11_Tokyo_Metropolitan   (2011-09-29)
Revision as of 15:30, 5 October 2011 by Naoto (Talk | contribs)

TetR generator (J23100-B0031-R0040-B0015)

TetR generator (J23100-B0031-R0040-B0015).


Repress expression of GFP by Anti-killing device

We confirm whether Anti-killing Device is working or not. We transform Anti-killing Device into E.coli which have GFP generator(I13522:Ptet-B0034-GFP-B0015) and observe its florescence. Figure2 is a result of this experiment. Left is E.coli transformed Anti-killing Device, right is control. Although not perfectly repressed GFP expression, we confirmed Anti-killing Device is working. Figure5 is difference of GFP florescence by comparing with absorbance of 525nm. For perfectly repressed expression cording under Ptet, RBS is needed to be more strong(ex.B0034).In addition, we should do more experience to get quantitative date.

<img src="Tokyo_Metropolitan_Killing2.png" width="300">
Figure5: repress expression of GFP by Anti-killing device

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None