Device
Part:BBa_K533002:Design
Designed by: ZHANG Yunxiao Group: iGEM11_Tsinghua (2011-09-29)
OmpA-HIV protease site-SH3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 857
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The HIV protease site is embedded in the protein so that a proteolytic cleavage can release the binding cassette.
Source
OmpA is from BL21(DE3) E. Coli genomic DNA. SH3 is from human Grb2 protein. HIV protease site is from a former part.