Device

Part:BBa_K533003

Designed by: ZHANG Yunxiao   Group: iGEM11_Tsinghua   (2011-09-29)
Revision as of 14:57, 5 October 2011 by Wishyx (Talk | contribs)

OmpA-HIV aspartyl protease

OmpA is used to present HIV aspartyl protease outside the E. Coli membrane so that it can cut other proteins with the specific sequence outside in the media.


Usage and Biology

We tested the function of this part. Our part BBa_K533002 contained an HIV-protease site. After BBa_K533002 was bound to mCherry, we cleaved it with bacteria expressing this part.

Color Channel + E. coli expressing protease + E. coli without protease
mCherry Thuexp release p.png Thuexp release n.png
DAPI Thuexp release p d.png Thuexp release n d.png
merge Thuexp release p m.png Thuexp release n m.png

It shows that it works.

We further optimized the conditions for expression induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 936
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme/protease
//cds/membrane/extracellular
//plasmid/expression/t7
Parameters
negative_regulatorsTemperature < 20 centigrade for efficient protein folding
positive_regulatorsIPTG, 20mM