Device

Part:BBa_K533003

Designed by: ZHANG Yunxiao   Group: iGEM11_Tsinghua   (2011-09-29)
Revision as of 14:20, 29 September 2011 by Registry (Talk | contribs)

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OmpA-HIV aspartyl protease

OmpA is used to present HIV aspartyl protease outside the E. Coli membrane so that it can cut other proteins with the specific sequence outside in the media.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 936
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme/protease
//cds/membrane/extracellular
//plasmid/expression/t7
Parameters
negative_regulatorsTemperature < 20 centigrade for efficient protein folding
positive_regulatorsIPTG, 20mM