Part:BBa_K533003:Experience
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how you used this part and how it worked out.
Applications of BBa_K533003
Like BBa_I712667, it can specifically cleave proteins in the peptide. The difference is that it is expressed as a membrane protein, which enables it to cut target proteins in the media.
We tested function of the protease with OmpA-HIV protese site-SH3, our part BBa_K533002.
E. coli expressing OmpA-HIV protease site-SH3 were first mixed up in multi-proline mCherry, to give it the red fluorescence marker. After washing with distilled water twice, the remaining bacteria were mixed with E. coli expressing OmpA-HIV protease at room temperature for 30 minutes to release the cargo.
After washing with distilled water twice, bacteria were fixed on a slip and stained with DAPI.
| Color Channel | + E. coli expressing protease | + E. coli without protease |
| mCherry | 
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| DAPI | 
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| merge | 
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User Reviews
UNIQ7b2a32dc7e8218f7-partinfo-00000000-QINU UNIQ7b2a32dc7e8218f7-partinfo-00000001-QINU