Part:BBa_K598001

Theophylline Responsive Riboswitch 1G1 with Engineered RBS+GFP generator

This is a GFP generator regulated by theophylline responsive riboswitch. mRNA with a theophylline riboswitch in it responds to theophylline concerntration, producing different fluorescence strength of GFP. Regulated by different promoters, it would be used to demonstrate theophylline responsive curve, providing data for modeling.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 71
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 730
  

Background

Riboswitches are structural domains embedded within the non-coding region of mRNA, performing as RNA-based genetic control elements that regulate gene expression in a ligand-dependent manner through direct, small molecule-RNA interactions without assistance of proteins. Over past several years, a number of natural riboswitches have been reported in literatures, sensing small molecules including coenzyme B1 and B12, FMN, SAM, lysine, guanine, adenine, TPP and theophylline [1]. Riboswitches are typically comprised of an aptamer domain, which recognizes a specific ligand with high specificity and affinity folding as a binding pocket [2]. The binding induces structural reorganization of aptamer and consequently influences the folding of adjoining expression platform, which varies due to different mechanisms of regulation.