Plasmid

Part:BBa_K524005:Design

Designed by: HO Yuan Heng Trevor   Group: iGEM11_HKUST   (2011-10-04)
Revision as of 06:12, 4 October 2011 by Registry (Talk | contribs)

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pToolkit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1215
    Illegal EcoRI site found at 2724
    Illegal PstI site found at 2219
    Illegal PstI site found at 2466
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1215
    Illegal EcoRI site found at 2724
    Illegal PstI site found at 2219
    Illegal PstI site found at 2466
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1215
    Illegal EcoRI site found at 2724
    Illegal BamHI site found at 1154
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1215
    Illegal EcoRI site found at 2724
    Illegal PstI site found at 2219
    Illegal PstI site found at 2466
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1215
    Illegal EcoRI site found at 2724
    Illegal PstI site found at 2219
    Illegal PstI site found at 2466
    Illegal AgeI site found at 989
    Illegal AgeI site found at 2646
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4422
    Illegal SapI site found at 971


Design Notes

We construct the pToolkit by replacing its heat sensitive replication origin oriR101&RepA101 with pir gene regulated replication origin ori-gamma.


Source

The backbone of the pToolkit, including the lambda-red swapping system and the Ampicilin Resistance Gene comes from pKD46. The replication origin of the pToolkit is ori-gamma, from the relication origin R6K.

References