Generator

Part:BBa_K524004

Designed by: HO Yuan Heng Trevor   Group: iGEM11_HKUST-Hong_Kong   (2011-10-03)
Revision as of 19:56, 3 October 2011 by Hyht2011 (Talk | contribs)

pir gene

The pir gene codes for the pi protein. Initiation of replication at ori-gamma requires the pi protein in trans. Doubling the concentration of pi protein would, however, effectively shut down the replication. Expression of pi protein is autogenously regulated.

Usage and Biology

The presence of pir gene can allow maintenance of plasmids with R6K origins as their sole origin of replication.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 438
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None