Generator

Part:BBa_K524004:Design

Designed by: HO Yuan Heng Trevor   Group: iGEM11_HKUST-Hong_Kong   (2011-10-03)
Revision as of 01:16, 4 October 2011 by Hyht2011 (Talk | contribs) (→‎Design Notes)

pir gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 438
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Homologous sites in primers designed by researchers to clone the pir gene into the E. coli BW25141 genome were used to clone out and standardize the pir gene. This is to avoid truncation of pir gene, and therefore junk sequences of 154 base pairs can be found downstream of the pir CDS.

Source

Cloned out and standardized from the genome of strain BW25141 (courtesy of The Coli Genetic Stock Center).

References