Composite

Part:BBa_K567001

Designed by: Chunying Li and Yunfeng Ruan   Group: iGEM11_SJTU-BioX-Shanghai   (2011-09-29)
Revision as of 08:10, 29 September 2011 by Registry (Talk | contribs)

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lacI-Ptrc-tRNA(Arg)

The tRNA(Arg) is under the control of promoter tac. tRNA(Arg) expression is induced by 0.5mM IPTG when the OD600 of the culture reaches 0.3.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1845
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1845
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1845
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1845
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2026


[edit]
Categories
Parameters
chassisEscherichia coli
functionIPTG induced tRNA expression
ligandsIsopropyl β-D-1-Thiogalactopyranoside
n/alacI-Ptrc-tRNA(Arg)