Reporter

Part:BBa_K598001

Designed by: Siyang HAO, Tong MU, Zheng LUO, Mengqi ZHANG   Group: iGEM11_Peking_R   (2011-09-27)
Revision as of 09:19, 5 October 2011 by Mutong (Talk | contribs)

Theophylline Responsive Riboswitch 1G1 with Engineered RBS+GFP generator

This is a GFP generator regulated by theophylline responsive riboswitch. mRNA with a theophylline riboswitch in it responds to theophylline concerntration, producing different fluorescence strength of GFP. Regulated by different promoters, it would be used to demonstrate theophylline responsive curve, providing data for modeling.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 71
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 730


Background

[edit]
Categories
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
//regulation/positive
//rna/riboswitch
regulator
Parameters
chassisE. coli DH5α
emit511 nm
excite501 nm
ligandstheophylline
n/aTheophylline Responsive Riboswitch 1G1 with Engineered RBS+GFP generator
outputGFP
rbsrobust
resistanceChloramphenicol