Reporter

Part:BBa_K598001

Designed by: Siyang HAO, Tong MU, Zheng LUO, Mengqi ZHANG   Group: iGEM11_Peking_R   (2011-09-27)
Revision as of 08:06, 5 October 2011 by Tani (Talk | contribs)

Theophylline Responsive Riboswitch 1G1 with Engineered RBS+GFP generator

This is a GFP generator with a theophylline responsive riboswitch sequence. The mRNA with a theophylline riboswitch in it would respond to theophylline concerntration, producing different amount of GFP. Under the control of different promoters, it could be used to demonstrate the theophylline responsive curve, providing data for modeling. The theophylline riboswitch sequence could also be used to regulate the translation of other genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 71
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 730


[edit]
Categories
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
//regulation/positive
//rna/riboswitch
regulator
Parameters
chassisE. coli DH5α
emit511 nm
excite501 nm
ligandstheophylline
n/aTheophylline Responsive Riboswitch 1G1 with Engineered RBS+GFP generator
outputGFP
rbsrobust
resistanceChloramphenicol