Device
Part:BBa_K598026:Design
Designed by: Shuobing CHEN, Tong MU Group: iGEM11_Peking_R (2011-09-28)
AND gate (BBa_K228258) regulated by TPP riboswitch 1.20 (BBa_K598004)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1274
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2144
Illegal BamHI site found at 1214 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1976
Illegal AgeI site found at 1320 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1323
Design Notes
The RBS of the TPP ribozyme is the native one.
Source
The AND gate is designed by Peking iGEM 2009 team