Reporter

Part:BBa_K524002

Designed by: Trevor Y. H. Ho   Group: iGEM11_HKUST-Hong_Kong   (2011-09-28)
Revision as of 19:40, 3 October 2011 by Hyht2011 (Talk | contribs) (→‎Usage and Biology)

pLac + RBS+ split sfGFP11 + double terminator

The split sfGFP1-10 was constructed from an pBAD driven sfGFP BBa_I746908. CDS of sfGFP includes amino acid residues 215-238 of sfGFP. Split sfGFPs at amino acid residues 214 and 215 have been reported to undergo spontaneous complementation to give green fluorescence.

Usage and Biology

This product of this biobrick should complement with the product of biobrick sfGFP1-10 BBa_K524001 to give green fluorescence under UV.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None